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Image Search Results
Journal: BMC Cancer
Article Title: Therapeutic potential of a novel prodrug of green tea extract in induction of apoptosis via ERK/JNK and Akt signaling pathway in human endometrial cancer
doi: 10.1186/s12885-020-07455-3
Figure Lengend Snippet: Apoptotic and microvessel development in endometrial carcinoma xenograft tumors after ProEGCG treatment. Haemotoxylin & eosin staining, TUNEL assay, caspase-3 and CD34 in ( a ) Rl95–2 and ( b ) AN3 CA xenograft tumors were analyzed by immunohistochemistry (magnification 200x). c, d The expression levels of CD34-postive cells were quantified. e Hierarchical clustering shows differential regulation of BAG1, NOD1 and NAIP (pink tree branch, yellow box) after EGCG and ProEGCG treatments in human EC cell xenografts. f Quantitative analysis by real-time PCR validated the differential expression of NOD1, NAIP and BAG1 after EGCG and ProEGCG treatment in human EC cell lines. Data are presented as mean ± S.E.M. Significant differences from control: * P < 0.05 and ** P < 0.01
Article Snippet: TaqMan probes of human NOD1 (Hs01036720_m1), NAIP (Hs03037952_m1), BAG1 (
Techniques: Staining, TUNEL Assay, Immunohistochemistry, Expressing, Real-time Polymerase Chain Reaction, Quantitative Proteomics, Control
Journal: Cellular and Molecular Life Sciences: CMLS
Article Title: Proteostasis network response to environmental chronic stress: linking survival to protein aggregation in a human neuroblastoma cellular model
doi: 10.1007/s00018-025-05884-6
Figure Lengend Snippet: Analysis of the BAG1/BAG3 triage system. a Western blot analysis of the expression of BAG1 in the RIPA-soluble protein fraction after 24 h ( n = 3) and b 72 h ( n = 4). c Analysis of the expression of soluble BAG3 after 24 h ( n = 4) and d 72 h ( n = 4). e. Analysis of BAG3 in the insoluble fraction after 72 h of treatment ( n = 3). Data were normalized against Vinculin as loading control. For the insoluble fraction, the same amount of proteins quantified in the respective soluble fractions were considered. Data are expressed as fold increase ± S.E.M and compared to normalized control, shown as dashed line. Statistical significance was determined after one-way ANOVA with Dunnet’s multiple comparison test of each treatment against control. *** p < 0.001; ** p < 0.01; * p < 0.05
Article Snippet: Primary antibodies utilized were: PARP-1, Cell Signaling Technology, 9542; BiP/Grp78, Abcam, ab32618; p-eIF2⍺, Cell Signaling Technology, 9721; eIF2⍺, Cell Signaling Technology, 9722; Ubiquitin, antibodies.com, A85455; BAG3, Invitrogen, MA5-32706;
Techniques: Western Blot, Expressing, Control, Comparison